Drosophila Defined Medium (DDM2)
| Reagent | Distributor and catalog number | Final concentration |
|---|---|---|
| DMEM/Ham's F-12 | Thermo Fisher 10565018 | Basal medium |
| HEPES | Thermo Fisher 15630080 | 15 mm |
| Putrescine | Nacalai Tesque 29428-01 | 100 µm |
| Insulin/transferrin/sodium selenite (ITS, 100×) | Sigma-Aldrich I13146 | 1× (10 mM insulin, 5.5 µg/mL transferrin, 5 ng/mL sodium selenite) |
| Progesterone | Nacalai Tesque 28921-51 | 20 ng/mL |
| Penicillin/streptomycin | Thermo Fisher 15140122 | 1× (100 U/mL) |
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Sterilize the solution using a 0.2-µm syringe filter. DDM2 can be made in advance and stored for up to 2 wk at 4°C or for up to 1 mo at −20°C.
