TNT Wash Buffer for Nothobranchius
| Reagent stock | Volume required for 50 mL | Final concentration |
|---|---|---|
| Tris HCl (Roche 10812846001), 1 m, pH 9.5 |
5 mL | 100 mm |
| NaCl, 5 m | 1.5 mL | 150 mm |
| Tween-20 (VWR 97062-332) 10% (v/v) stock |
250 µL | 0.05% |
| H2O | Adjust to 50 mL |
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Sterilization is not necessary. Store indefinitely at room temperature. Discard if a precipitate forms.
