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A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos

Brook T. Chernet and
Michael Levin

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067694

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Preparation of Vesicular Stomatitis Virus-G (VSV-G) Conjugate and Its Use in Gene Transfer

Atsushi Miyanohara

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068528

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In Vitro Transfection with Plasmid DNA Using Polylysine Copolymers

Sung Wan Kim

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068627

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Measuring Resting Membrane Potential Using the Fluorescent Voltage Reporters DiBAC₄(3) and CC2-DMPE

Dany S. Adams and
Michael Levin

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067702

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Presynaptic Imaging of Projection Fibers by In Vivo Injection of Dextran-Conjugated Calcium Indicators

Stephan D. Brenowitz and
Wade G. Regehr

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068551

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Multiphoton Stimulation of Neurons and Spines

Hajime Hirase,
Volodymyr Nikolenko,
and Rafael Yuste

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068569

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Building an Imaging Chamber for In Vivo Imaging of Drosophila Larvae

Till F.M. Andlauer and
Stephan J. Sigrist

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068585

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In Vivo Imaging of the Drosophila Larval Neuromuscular Junction

Till F.M. Andlauer and
Stephan J. Sigrist

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068593

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Quantitative Analysis of Drosophila Larval Neuromuscular Junction Morphology

Till F.M. Andlauer and
Stephan J. Sigrist

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068601

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Microultrasound and Its Application to Longitudinal Studies of Mouse Eye Development and Disease

F. Stuart Foster and
Allison S. Brown

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068544

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Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems

Derek Toomre

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068668

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Transmission Electron Microscopy of Thin Sections of Drosophila: High-Pressure Freezing and Freeze-Substitution

Kent L. McDonald,
David J. Sharp,
and Wayne Rickoll

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068403

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Transmission Electron Microscopy of Thin Sections of Drosophila: Conventional Chemical Fixation of Embryos Using Trialdehyde

Kent L. McDonald,
David J. Sharp,
and Wayne Rickoll

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068411

TOPIC INTRODUCTIONS

General Principles for Measuring Resting Membrane Potential and Ion Concentration Using Fluorescent Bioelectricity Reporters

Quantifying the Bicoid Morphogen Gradient in Living Fly Embryos

In Vivo Imaging of Drosophila Larval Neuromuscular Junctions to Study Synapse Assembly

Cellular Imaging Using Total Internal Reflection Fluorescence Microscopy: Theory and Instrumentation

Microsatellite Markers for Linkage and Association Studies

INFORMATION PANELS

Polylysine Copolymers for Gene Delivery

Generating Live Cell Data Using Total Internal Reflection Fluorescence Microscopy

PROTOCOLS

A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos

Preparation of Vesicular Stomatitis Virus-G (VSV-G) Conjugate and Its Use in Gene Transfer

In Vitro Transfection with Plasmid DNA Using Polylysine Copolymers

Measuring Resting Membrane Potential Using the Fluorescent Voltage Reporters DiBAC₄(3) and CC2-DMPE

Presynaptic Imaging of Projection Fibers by In Vivo Injection of Dextran-Conjugated Calcium Indicators

Multiphoton Stimulation of Neurons and Spines

Building an Imaging Chamber for In Vivo Imaging of Drosophila Larvae

In Vivo Imaging of the Drosophila Larval Neuromuscular Junction

Quantitative Analysis of Drosophila Larval Neuromuscular Junction Morphology

Microultrasound and Its Application to Longitudinal Studies of Mouse Eye Development and Disease

Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems

Transmission Electron Microscopy of Thin Sections of Drosophila: High-Pressure Freezing and Freeze-Substitution

Transmission Electron Microscopy of Thin Sections of Drosophila: Conventional Chemical Fixation of Embryos Using Trialdehyde

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

General Principles for Measuring Resting Membrane Potential and Ion Concentration Using Fluorescent Bioelectricity Reporters

Quantifying the Bicoid Morphogen Gradient in Living Fly Embryos

In Vivo Imaging of Drosophila Larval Neuromuscular Junctions to Study Synapse Assembly

Cellular Imaging Using Total Internal Reflection Fluorescence Microscopy: Theory and Instrumentation

Microsatellite Markers for Linkage and Association Studies

INFORMATION PANELS

Polylysine Copolymers for Gene Delivery

Generating Live Cell Data Using Total Internal Reflection Fluorescence Microscopy

PROTOCOLS

A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos

Preparation of Vesicular Stomatitis Virus-G (VSV-G) Conjugate and Its Use in Gene Transfer

In Vitro Transfection with Plasmid DNA Using Polylysine Copolymers

Measuring Resting Membrane Potential Using the Fluorescent Voltage Reporters DiBAC4(3) and CC2-DMPE

Presynaptic Imaging of Projection Fibers by In Vivo Injection of Dextran-Conjugated Calcium Indicators

Multiphoton Stimulation of Neurons and Spines

Building an Imaging Chamber for In Vivo Imaging of Drosophila Larvae

In Vivo Imaging of the Drosophila Larval Neuromuscular Junction

Quantitative Analysis of Drosophila Larval Neuromuscular Junction Morphology

Microultrasound and Its Application to Longitudinal Studies of Mouse Eye Development and Disease

Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems

Transmission Electron Microscopy of Thin Sections of Drosophila: High-Pressure Freezing and Freeze-Substitution

Transmission Electron Microscopy of Thin Sections of Drosophila: Conventional Chemical Fixation of Embryos Using Trialdehyde

This Issue

Current Issue

From the cover

Measuring Resting Membrane Potential Using the Fluorescent Voltage Reporters DiBAC₄(3) and CC2-DMPE