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Subcapsular Transplantation of Tissue in the Kidney

Leonard D. Shultz,
Neal Goodwin,
Fumihiko Ishikawa,
Vishnu Hosur,
Bonnie L. Lyons,
and Dale L. Greiner

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot078089

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PiggyBac Transposon-Mediated Cellular Transgenesis in Mammalian Forebrain by In Utero Electroporation

Fuyi Chen,
Brady J. Maher,
and Joseph J. LoTurco

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073650

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Presynaptic Calcium Measurements Using Bulk Loading of Acetoxymethyl Indicators

Stephan D. Brenowitz and
Wade G. Regehr

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot081828

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Measuring the Steady-State Properties of Ca²⁺ Indicators with a Set of Calibrated [Ca²⁺] Solutions

Guido C. Faas and
Istvan Mody

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073270

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Measuring the Rate Constants of Ca²⁺ Indicators

Guido C. Faas and
Istvan Mody

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073288

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Collecting Data to Determine the Ca²⁺-Binding Properties of DM-Nitrophen and Proteins

Guido C. Faas and
Istvan Mody

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073296

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Assaying Caspase Activity In Vitro

Gavin P. McStay and
Douglas R. Green

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080283

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Preparation of Cytosolic Extracts and Activation of Caspases by Cytochrome c

Gavin P. McStay and
Douglas R. Green

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080275

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Detection of Caspase Activity Using Antibody-Based Techniques

Gavin P. McStay and
Douglas R. Green

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080291

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Detection of RNAs by 3′-End Labeling and RNase H Digestion

Timothy W. Nilsen

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080796

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Northern Blots for Small RNAs and MicroRNAs

Donald C. Rio

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080838

TOPIC INTRODUCTIONS

Measuring the Ca²⁺-Binding Kinetics of Proteins

Human Cancer Growth and Therapy in Immunodeficient Mouse Models

Fluorescence Correlation Spectroscopy: Principles and Applications

Two-Photon Imaging of Neural Activity in Awake Mobile Mice

PROTOCOLS

Subcapsular Transplantation of Tissue in the Kidney

PiggyBac Transposon-Mediated Cellular Transgenesis in Mammalian Forebrain by In Utero Electroporation

Presynaptic Calcium Measurements Using Bulk Loading of Acetoxymethyl Indicators

Measuring the Steady-State Properties of Ca²⁺ Indicators with a Set of Calibrated [Ca²⁺] Solutions

Measuring the Rate Constants of Ca²⁺ Indicators

Collecting Data to Determine the Ca²⁺-Binding Properties of DM-Nitrophen and Proteins

Assaying Caspase Activity In Vitro

Preparation of Cytosolic Extracts and Activation of Caspases by Cytochrome c

Detection of Caspase Activity Using Antibody-Based Techniques

Detection of RNAs by 3′-End Labeling and RNase H Digestion

Northern Blots for Small RNAs and MicroRNAs

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

Measuring the Ca2+-Binding Kinetics of Proteins

Human Cancer Growth and Therapy in Immunodeficient Mouse Models

Fluorescence Correlation Spectroscopy: Principles and Applications

Two-Photon Imaging of Neural Activity in Awake Mobile Mice

PROTOCOLS

Subcapsular Transplantation of Tissue in the Kidney

PiggyBac Transposon-Mediated Cellular Transgenesis in Mammalian Forebrain by In Utero Electroporation

Presynaptic Calcium Measurements Using Bulk Loading of Acetoxymethyl Indicators

Measuring the Steady-State Properties of Ca2+ Indicators with a Set of Calibrated [Ca2+] Solutions

Measuring the Rate Constants of Ca2+ Indicators

Collecting Data to Determine the Ca2+-Binding Properties of DM-Nitrophen and Proteins

Assaying Caspase Activity In Vitro

Preparation of Cytosolic Extracts and Activation of Caspases by Cytochrome c

Detection of Caspase Activity Using Antibody-Based Techniques

Detection of RNAs by 3′-End Labeling and RNase H Digestion

Northern Blots for Small RNAs and MicroRNAs

This Issue

Current Issue

From the cover

Measuring the Ca²⁺-Binding Kinetics of Proteins

Measuring the Steady-State Properties of Ca²⁺ Indicators with a Set of Calibrated [Ca²⁺] Solutions

Measuring the Rate Constants of Ca²⁺ Indicators

Collecting Data to Determine the Ca²⁺-Binding Properties of DM-Nitrophen and Proteins