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Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits

Naoki Yamawaki,
Benjamin A. Suter,
Ian R. Wickersham,
and Gordon M. G. Shepherd

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090084

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Reporter Gene Silencing Assays in Fission Yeast

Hugh P. Cam and
Simon Whitehall

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091512

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Analysis of Schizosaccharomyces pombe Heterochromatin-Associated Short Interfering RNAs

Ke Zhang

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091520

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Optimized CRISPR–Cas9 System for Genome Editing in Zebrafish

Charles E. Vejnar,
Miguel A. Moreno-Mateos,
Daniel Cifuentes,
Ariel A. Bazzini,
and Antonio J. Giraldez

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086850

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Preparation of Yeast DNA Sequencing Libraries

Katja Schwartz and
Gavin Sherlock

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088930

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Characterization of Protein–Protein Interactions Using Protein Microarrays

Corry Paul,
HeeSool Rho,
Johnathan Neiswinger,
and Heng Zhu

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087965

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Characterization of RNA-Binding Proteins Using Protein Microarrays

Guang Song,
Johnathan Neiswinger,
and Heng Zhu

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087973

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Characterization of Lipid–Protein Interactions Using Nonquenched Fluorescent Liposomal Nanovesicles and Yeast Proteome Microarrays

Kuan-Yi Lu,
Chien-Sheng Chen,
Johnathan Neiswinger,
and Heng Zhu

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087981

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Posttranslational Modification Assays on Functional Protein Microarrays

Johnathan Neiswinger,
Ijeoma Uzoma,
Eric Cox,
HeeSool Rho,
Jun Seop Jeong,
and Heng Zhu

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087999

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Detection of DNA Fragmentation in Apoptotic Cells by TUNEL

Lisa C. Crowley,
Brooke J. Marfell,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087221

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Measuring the DNA Content of Cells in Apoptosis and at Different Cell-Cycle Stages by Propidium Iodide Staining and Flow Cytometry

Lisa C. Crowley,
Grace Chojnowski,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087247

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Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot093344

TOPIC INTRODUCTIONS

Optimization Strategies for the CRISPR–Cas9 Genome-Editing System

High-Throughput Yeast Strain Sequencing

Protein Microarrays: Flexible Tools for Scientific Innovation

PROTOCOLS

Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits

Reporter Gene Silencing Assays in Fission Yeast

Analysis of Schizosaccharomyces pombe Heterochromatin-Associated Short Interfering RNAs

Optimized CRISPR–Cas9 System for Genome Editing in Zebrafish

Preparation of Yeast DNA Sequencing Libraries

Characterization of Protein–Protein Interactions Using Protein Microarrays

Characterization of RNA-Binding Proteins Using Protein Microarrays

Characterization of Lipid–Protein Interactions Using Nonquenched Fluorescent Liposomal Nanovesicles and Yeast Proteome Microarrays

Posttranslational Modification Assays on Functional Protein Microarrays

Detection of DNA Fragmentation in Apoptotic Cells by TUNEL

Measuring the DNA Content of Cells in Apoptosis and at Different Cell-Cycle Stages by Propidium Iodide Staining and Flow Cytometry

Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps

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