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Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing

Yuichiro Miyaoka,
Amanda H. Chan,
and Bruce R. Conklin

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086801

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Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells

Andrew J.R. Plested and
Jelena Baranovic

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091652

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Fluorescent Labeling of Yeast Cell Wall Components

Hiroki Okada and
Yoshikazu Ohya

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085241

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Assay for Spore Wall Integrity Using a Yeast Predator

Hiroki Okada,
Aaron M. Neiman,
and Yoshikazu Ohya

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085258

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Local and Acute Disruption of the Yeast Cell Surface

Keiko Kono,
Hiroki Okada,
and Yoshikazu Ohya

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085266

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Synchronizing Progression of Schizosaccharomyces pombe Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release

Iain M. Hagan,
Agnes Grallert,
and Viesturs Simanis

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091256

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Synchronizing Progression of Schizosaccharomyces pombe Cells from G₂ through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release

Iain M. Hagan,
Agnes Grallert,
and Viesturs Simanis

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091264

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Measuring Survival of Adherent Cells with the Colony-Forming Assay

Lisa C. Crowley,
Melinda E. Christensen,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087171

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Measuring Survival of Hematopoietic Cancer Cells with the Colony-Forming Assay in Soft Agar

Lisa C. Crowley and
Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087189

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Selecting Female Mice in Estrus and Checking Plugs

Richard Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
and Andras Nagy

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot092387

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Determining the Stage of the Estrous Cycle in Female Mice by Vaginal Smear

Gabriel Gonzalez

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot094474

TOPIC INTRODUCTIONS

Data Formats in Bioinformatics

Detecting Single-Nucleotide Substitutions Induced by Genome Editing

Single-Channel Recording of Ligand-Gated Ion Channels

Examination and Disruption of the Yeast Cell Wall

PROTOCOLS

Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing

Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells

Fluorescent Labeling of Yeast Cell Wall Components

Assay for Spore Wall Integrity Using a Yeast Predator

Local and Acute Disruption of the Yeast Cell Surface

Synchronizing Progression of Schizosaccharomyces pombe Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release

Synchronizing Progression of Schizosaccharomyces pombe Cells from G₂ through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release

Measuring Survival of Adherent Cells with the Colony-Forming Assay

Measuring Survival of Hematopoietic Cancer Cells with the Colony-Forming Assay in Soft Agar

Selecting Female Mice in Estrus and Checking Plugs

Determining the Stage of the Estrous Cycle in Female Mice by Vaginal Smear

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

Data Formats in Bioinformatics

Detecting Single-Nucleotide Substitutions Induced by Genome Editing

Single-Channel Recording of Ligand-Gated Ion Channels

Examination and Disruption of the Yeast Cell Wall

PROTOCOLS

Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing

Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells

Fluorescent Labeling of Yeast Cell Wall Components

Assay for Spore Wall Integrity Using a Yeast Predator

Local and Acute Disruption of the Yeast Cell Surface

Synchronizing Progression of Schizosaccharomyces pombe Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release

Synchronizing Progression of Schizosaccharomyces pombe Cells from G2 through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release

Measuring Survival of Adherent Cells with the Colony-Forming Assay

Measuring Survival of Hematopoietic Cancer Cells with the Colony-Forming Assay in Soft Agar

Selecting Female Mice in Estrus and Checking Plugs

Determining the Stage of the Estrous Cycle in Female Mice by Vaginal Smear

This Issue

Current Issue

From the cover

Synchronizing Progression of Schizosaccharomyces pombe Cells from G₂ through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release